Partial neurorescue effects of DHA following a 6-OHDA lesion of the mouse dopaminergic system.

TitlePartial neurorescue effects of DHA following a 6-OHDA lesion of the mouse dopaminergic system.
Publication TypeJournal Article
Year of Publication2016
AuteursCoulombe, K, Saint-Pierre, M, Cisbani, G, St-Amour, I, Gibrat, C, Giguère-Rancourt, A, Calon, F, Cicchetti, F
JournalJ Nutr Biochem
Volume30
Pagination133-42
Date Published2016 Apr
ISSN1873-4847
KeywordsAnimals, Docosahexaenoic Acids, Mice, Mice, Inbred C57BL, Motor Activity, Oxidopamine
Abstract

Pre-clinical data collected in mouse models of Parkinson's disease (PD) support the neuroprotective potential of omega-3 polyunsaturated fatty acids (n-3 PUFA)-enriched diet on the dopaminergic (DAergic) system. In this study, we investigated the effects of an n-3 PUFA-rich diet using a neurorescue/neurorestorative paradigm. C57BL/6 adult mice were submitted to a striatal stereotaxic injection of the neurotoxin 6-hydroxydopamine (6-OHDA) to induce striatal DAergic denervation and subsequent nigral DAergic cell loss. Three weeks post-lesion, mice received either a docosahexaenoic acid (DHA)-enriched or a control diet for a period of 6 weeks. HPLC analyses revealed a 111% post-lesion increase in striatal dopamine levels in the DHA-fed animals compared to controls (ctrl, P<0.05), although no improvement in the motor behavior was observed. DHA treatment led to a 89% rise in tyrosine-hydroxylase (TH)-immunoreactive terminals within the striatum (P<0.05) in lesioned animals. Despite the fact that DHA did not change the number of TH+ neurons in the substantia nigra pars compacta (SNpc), morphological analyses revealed an increased in perimeters (+7%) and areas (+21%) of DAergic cell bodies in treated animals. Collectively, our results suggest that DHA induces a partial neurorescue/neurorestoration of the DAergic system and support further studies to investigate the potential of a diet-based intervention, or at least the combination of such approach, to current treatments in PD.

DOI10.1016/j.jnutbio.2015.11.014
Alternate JournalJ. Nutr. Biochem.
PubMed ID27012630
Grant List / / Canadian Institutes of Health Research / Canada