Mitochondrial Dysfunction in Schizophrenia: Determination of Mitochondrial Respiratory Activity in a Two-Hit Mouse Model.

TitleMitochondrial Dysfunction in Schizophrenia: Determination of Mitochondrial Respiratory Activity in a Two-Hit Mouse Model.
Publication TypeJournal Article
Year of Publication2016
AuteursMonpays, C, Deslauriers, J, Sarret, P, Grignon, S
JournalJ Mol Neurosci
Volume59
Issue4
Pagination440-51
Date Published2016 Aug
ISSN1559-1166
KeywordsAnimals, Cell Respiration, Corpus Striatum, Electron Transport Complex I, Electron Transport Complex II, Female, Male, Mice, Mice, Inbred C57BL, Mitochondria, Oxidative Stress, Prefrontal Cortex, Protein Carbonylation, Schizophrenia, Sex Factors, Thioctic Acid
Abstract

Schizophrenia is a chronic mental illness in which mitochondrial dysfunction has been suggested. Our laboratory recently developed a juvenile murine two-hit model (THM) of schizophrenia based on the combination of gestational inflammation, followed by juvenile restraint stress. We previously reported that relevant behaviors and neurochemical disturbances, including oxidative stress, were reversed by the antioxidant lipoic acid (LA), thereby pointing to the central role played by oxidative abnormalities and prompting us to investigate mitochondrial function. Mitochondrial activity was determined with the MitoXpress® commercial kit in two schizophrenia-relevant regions (prefrontal cortex (PFC) and striatum). Measurements were performed in state 3, with substrates for complex I- and complex II-induced respiratory activity (IRA). We observed an increase in complex I IRA in the PFC and striatum in both sexes but an increase in complex II activity only in males. LA treatment prevented this increase only in complex II IRA in males. Expression levels of the different respiratory chain complexes, as well as fission/fusion proteins and protein carbonylation, were unchanged. In conclusion, our juvenile schizophrenia THM shows an increase in mitochondrial activity reversed by LA, specifically in complex II IRA in males. Further investigations are required to determine the mechanisms of these modifications.

DOI10.1007/s12031-016-0746-3
Alternate JournalJ. Mol. Neurosci.
PubMed ID27034067