Methylation profiles of IL33 and CCL26 in bronchial epithelial cells are associated with asthma.

TitleMethylation profiles of IL33 and CCL26 in bronchial epithelial cells are associated with asthma.
Publication TypeJournal Article
Year of Publication2018
AuteursLarouche, M, Gagné-Ouellet, V, Boucher-Lafleur, A-M, Larose, M-C, Plante, S, Madore, A-M, Laviolette, M, Flamand, N, Chakir, J, Laprise, C
JournalEpigenomics
Volume10
Issue12
Pagination1555-1568
Date Published2018 Dec
ISSN1750-192X
KeywordsAdult, Alleles, Asthma, Chemokine CCL26, DNA Methylation, Eosinophils, Epigenomics, Epithelial Cells, Female, Gene Expression Regulation, Haplotypes, Humans, Interleukin-1 Receptor-Like 1 Protein, Interleukin-33, Lung, Male, Middle Aged, Mutation, Promoter Regions, Genetic, Young Adult
Abstract

AIM: This study aimed to characterize DNA methylation (DNA-me) in promoter region of IL33, IL1RL1 and CCL26 in asthma and their impacts on transcriptional activity in bronchial epithelial cells (BECs).PATIENTS & METHODS: We performed bis-pyrosequencing, quantitative real-time PCR and sequencing in BECs from ten asthmatic and ten control individuals.RESULTS: We detected lower DNA-me levels of IL33 and CCL26 in asthmatic than control BECs. No correlation was found between methylation and expression levels. Interestingly, carriers of a mutative allele in a haplotype within the promoter of IL33 had a lower IL33 DNA-me level and CCL26 gene expression correlated with eosinophil count.CONCLUSION: These findings highlight the importance of investigating both epigenetic and genetic mechanisms in understanding the epithelial immune response in asthma.

DOI10.2217/epi-2018-0044
Alternate JournalEpigenomics
PubMed ID30468398
Grant List / / CIHR / Canada