Local assessment of myelin health in a multiple sclerosis mouse model using a 2D Fourier transform approach.

TitleLocal assessment of myelin health in a multiple sclerosis mouse model using a 2D Fourier transform approach.
Publication TypeJournal Article
Year of Publication2013
AuteursBégin, S, Bélanger, E, Laffray, S, Aubé, B, Chamma, É, Bélisle, J, Lacroix, S, De Koninck, Y, Côté, DC
JournalBiomed Opt Express
Volume4
Issue10
Pagination2003-14
Date Published2013
ISSN2156-7085
Abstract

We present an automated two-dimensional Fourier transform (2D-FT) approach to analyze the local organization of myelinated axons in the spinal cord. Coherent anti-Stokes Raman scattering (CARS) microscopy was used to observe lesions in a commonly used animal model of multiple sclerosis (MS), experimental autoimmune encephalomyelitis (EAE). A 2D-FT was applied on the CARS images to find the average orientation and directional anisotropy of the fibers within contiguous image domains. We introduce the corrected correlation parameter (CCP), a measure of the correlation between orientations of adjacent domains. We show that in the EAE animal model of MS, the CCP can be used to quantify the degree of organization/disorganization in the myelin structure. This analysis was applied to a large image dataset from animals at different clinical scores and we show that some descriptors of the CCP probability density function are strongly correlated with the clinical scores. This procedure, compatible with live animal imaging, has been developed to perform local in situ evaluation of myelinated axons afflicted by EAE.

DOI10.1364/BOE.4.002003
Alternate JournalBiomed Opt Express
PubMed ID24156060
PubMed Central IDPMC3799662