Leukotriene D4 and interleukin-13 cooperate to increase the release of eotaxin-3 by airway epithelial cells.

TitleLeukotriene D4 and interleukin-13 cooperate to increase the release of eotaxin-3 by airway epithelial cells.
Publication TypeJournal Article
Year of Publication2012
AuteursProvost, V, Langlois, A, Chouinard, F, Rola-Pleszczynski, M, Chakir, J, Flamand, N, Laviolette, M
JournalPLoS One
Volume7
Issue8
Paginatione43544
Date Published2012
ISSN1932-6203
KeywordsAsthma, Bronchi, Chemokine CCL24, Chemokine CCL26, Chemokines, CC, Cysteine, Enzyme-Linked Immunosorbent Assay, Epithelial Cells, Flow Cytometry, Gene Expression Regulation, Humans, Inflammation, Interleukin-13, Interleukin-4, Kinetics, Leukotriene D4, Leukotrienes, Models, Biological, Recombinant Proteins, Th2 Cells
Abstract

INTRODUCTION: Airway epithelial cells play a central role in the physiopathology of asthma. They release eotaxins when treated with T(H)2 cytokines such as interleukin (IL)-4 or IL-13, and these chemokines attract eosinophils and potentiate the biosynthesis of cysteinyl leukotrienes (cysLTs), which in turn induce bronchoconstriction and mucus secretion. These effects of cysLTs mainly mediated by CysLT(1) and CysLT(2) receptors on epithelial cell functions remain largely undefined. Because the release of inflammatory cytokines, eotaxins, and cysLTs occur relatively at the same time and location in the lung tissue, we hypothesized that they regulate inflammation cooperatively rather than redundantly. We therefore investigated whether cysLTs and the T(H)2 cytokines would act in concert to augment the release of eotaxins by airway epithelial cells.METHODS: A549 cells or human primary bronchial epithelial cells were incubated with or without IL-4, IL-13, and/or LTD(4). The release of eotaxin-3 and the expression of cysLT receptors were assessed by ELISA, RT-PCR, and flow cytometry, respectively.RESULTS: IL-4 and IL-13 induced the release of eotaxin-3 by airway epithelial cells. LTD(4) weakly induced the release of eotaxin-3 but clearly potentiated the IL-13-induced eotaxin-3 release. LTD(4) had no effect on IL-4-stimulated cells. Epithelial cells expressed CysLT(1) but not CysLT(2). CysLT(1) expression was increased by IL-13 but not by IL-4 and/or LTD(4). Importantly, the upregulation of CysLT(1) by IL-13 preceded eotaxin-3 release.CONCLUSIONS: These results demonstrate a stepwise cooperation between IL-13 and LTD(4). IL-13 upregulates CysLT(1) expression and consequently the response to cysLTs This results in an increased release of eotaxin-3 by epithelial cells which at its turn increases the recruitment of leukocytes and their biosynthesis of cysLTs. This positive amplification loop involving epithelial cells and leukocytes could be implicated in the recruitment of eosinophils observed in asthmatics.

DOI10.1371/journal.pone.0043544
Alternate JournalPLoS ONE
PubMed ID22952702
PubMed Central IDPMC3432028
Grant List / / Canadian Institutes of Health Research / Canada