Expression of membrane type-4 matrix metalloproteinase (metalloproteinase-17) by human eosinophils.

TitleExpression of membrane type-4 matrix metalloproteinase (metalloproteinase-17) by human eosinophils.
Publication TypeJournal Article
Year of Publication2003
AuteursGauthier, M-C, Racine, C, Ferland, C, Flamand, N, Chakir, J, Tremblay, GM, Laviolette, M
JournalInt J Biochem Cell Biol
Volume35
Issue12
Pagination1667-73
Date Published2003 Dec
ISSN1357-2725
KeywordsCells, Cultured, Eosinophils, Humans, Immunohistochemistry, Matrix Metalloproteinases, Matrix Metalloproteinases, Membrane-Associated, Metalloendopeptidases, Nasal Polyps, RNA, Messenger, Tumor Necrosis Factor-alpha
Abstract

Circulating eosinophils need proteinases to mediate a spatially limited and orientated digestion of the extracellular matrix and to migrate into tissue. Moreover, proteinases are likely involved in tissue remodeling, a crucial feature of chronic diseases including asthma. Eosinophils express matrix metalloproteinase (MMP)-9, which is increased upon stimulation with TNF-alpha. Other MMPs, the membrane type (MT)-MMPs, likely play a major role in cell invasion and tissue remodeling. MT4-MMP was identified in peripheral blood leukocyte preparations, but it is not known whether eosinophils express MT4-MMP. We investigated the expression of MT4-MMP and its modulation by TNF-alpha in purified human blood eosinophils. The constitutive expression of MT4-MMP mRNA was detected by RT-PCR in unstimulated eosinophils, lymphocytes, and monocytes, but not neutrophils. Stimulation of eosinophils with TNF-alpha increased MT4-MMP mRNA expression. This effect appeared at 4h and reached a maximum at 8h of incubation. MT4-MMP protein was detected in freshly isolated blood eosinophils by Western blotting and immunocytochemistry. TNF-alpha increased expression of the MT4-MMP protein. MT4-MMP protein was also detected in nasal polyp eosinophils by immunohistochemistry. In conclusion, eosinophils constitutively express MT4-MMP, which is increased upon stimulation with TNF-alpha. Consequently, MT4-MMP may be directly involved in the degradation of extracellular matrix components and/or modulate the activity of other proteins implicated in eosinophil migration and tissue remodeling.

DOI10.1016/s1357-2725(03)00136-5
Alternate JournalInt. J. Biochem. Cell Biol.
PubMed ID12962706