Characterization of the honeybee AmNaV1 channel and tools to assess the toxicity of insecticides.

TitleCharacterization of the honeybee AmNaV1 channel and tools to assess the toxicity of insecticides.
Publication TypeJournal Article
Year of Publication2015
AuteursGosselin-Badaroudine, P, Moreau, A, Delemotte, L, Cens, T, Collet, C, Rousset, M, Charnet, P, Klein, ML, Chahine, M
JournalSci Rep
Volume5
Pagination12475
Date Published2015 Jul 23
ISSN2045-2322
KeywordsAmino Acid Sequence, Animals, Bees, Binding Sites, Insecticides, Ion Channel Gating, Molecular Docking Simulation, Molecular Sequence Data, Protein Binding, Protein Conformation, Sodium Channel Blockers, Toxicity Tests, Voltage-Gated Sodium Channels
Abstract

Pollination is important for both agriculture and biodiversity. For a significant number of plants, this process is highly, and sometimes exclusively, dependent on the pollination activity of honeybees. The large numbers of honeybee colony losses reported in recent years have been attributed to colony collapse disorder. Various hypotheses, including pesticide overuse, have been suggested to explain the disorder. Using the Xenopus oocytes expression system and two microelectrode voltage-clamp, we report the functional expression and the molecular, biophysical, and pharmacological characterization of the western honeybee's sodium channel (Apis Mellifera NaV1). The NaV1 channel is the primary target for pyrethroid insecticides in insect pests. We further report that the honeybee's channel is also sensitive to permethrin and fenvalerate, respectively type I and type II pyrethroid insecticides. Molecular docking of these insecticides revealed a binding site that is similar to sites previously identified in other insects. We describe in vitro and in silico tools that can be used to test chemical compounds. Our findings could be used to assess the risks that current and next generation pesticides pose to honeybee populations.

DOI10.1038/srep12475
Alternate JournalSci Rep
PubMed ID26202396
PubMed Central IDPMC4894402
Grant ListP01 GM055876 / GM / NIGMS NIH HHS / United States
P01 GM55876 / GM / NIGMS NIH HHS / United States