Characterization and cellular localization of human 5-lipoxygenase and its protein isoforms 5-LOΔ13, 5-LOΔ4 and 5-LOp12.

TitleCharacterization and cellular localization of human 5-lipoxygenase and its protein isoforms 5-LOΔ13, 5-LOΔ4 and 5-LOp12.
Publication TypeJournal Article
Year of Publication2017
AuteursBall, A-K, Beilstein, K, Wittmann, S, Sürün, D, Saul, MJ, Schnütgen, F, Flamand, N, Capelo, R, Kahnt, AS, Frey, H, Schaefer, L, Marschalek, R, Häfner, A-K, Steinhilber, D
JournalBiochim Biophys Acta Mol Cell Biol Lipids
Volume1862
Issue5
Pagination561-571
Date Published2017 May
ISSN1388-1981
KeywordsArachidonate 5-Lipoxygenase, Cell Nucleus, Cytosol, Gene Expression Regulation, Enzymologic, HEK293 Cells, Humans, Leukotrienes, Neutrophils, Phosphorylation, Protein Isoforms
Abstract

Human 5-lipoxygenase (5-LO-WT) initiates the leukotriene (LT) biosynthesis. LTs play an important role in diseases like asthma, atherosclerosis and in many types of cancer. In this study, we investigated the 5-LO isoforms 5-LO∆13, 5-LO∆4 and 5-LOp12, lacking the exons 13, 4 or a part of exon 12, respectively. We were able to detect the mRNA of the isoforms 5-LO∆13 and 5-LOp12 in B and T cell lines as well as in primary B and T cells and monocytes. Furthermore, we found that expression of 5-LO and particularly of the 5-LO∆13 and 5-LOp12 isoforms is increased in monocytes from patients with rheumatoid arthritis and sepsis. Confocal microscopy of HEK293T cells stably transfected with tagged 5-LO-WT and/or the isoforms revealed that 5-LO-WT is localized in the nucleus whereas all isoforms are located in the cytosol. Additionally, all isoforms are catalytically inactive and do not seem to influence the specific activity of 5-LO-WT. S271A mutation in 5-LO-WT and treatment of the cells with sorbitol or KN-93/SB203580 changes the localization of the WT enzyme to the cytosol. Despite colocalization with the S271A mutant, the isoforms did not affect LT biosynthesis. Analysis of the phosphorylation pattern of 5-LO-WT and all the isoforms revealed that 5-LOp12 and 5-LO∆13 are highly phosphorylated at Ser271 and 5-LOp12 at Ser523. Furthermore, coexpression of the isoforms inhibited or stimulated 5-LO-WT expression in transiently and stably transfected HEK293T cells suggesting that the isoforms have other functions than canonical LT biosynthesis.

DOI10.1016/j.bbalip.2017.02.015
Alternate JournalBiochim Biophys Acta Mol Cell Biol Lipids
PubMed ID28257804