Portrait of blood-derived extracellular vesicles in patients with Parkinson's disease.

TitlePortrait of blood-derived extracellular vesicles in patients with Parkinson's disease.
Publication TypeJournal Article
Year of Publication2019
AuthorsLamontagne-Proulx, J, St-Amour, I, Labib, R, Pilon, J, Denis, HL, Cloutier, N, Roux-Dalvai, F, Vincent, AT, Mason, SL, Williams-Gray, C, Duchez, A-C, Droit, A, Lacroix, S, Dupré, N, Langlois, M, Chouinard, S, Panisset, M, Barker, RA, Boilard, E, Cicchetti, F
JournalNeurobiol Dis
Volume124
Pagination163-175
Date Published2019 Apr
ISSN1095-953X
Abstract

The production of extracellular vesicles (EV) is a ubiquitous feature of eukaryotic cells but pathological events can affect their formation and constituents. We sought to characterize the nature, profile and protein signature of EV in the plasma of Parkinson's disease (PD) patients and how they correlate to clinical measures of the disease. EV were initially collected from cohorts of PD (n = 60; Controls, n = 37) and Huntington's disease (HD) patients (Pre-manifest, n = 11; manifest, n = 52; Controls, n = 55) - for comparative purposes in individuals with another chronic neurodegenerative condition - and exhaustively analyzed using flow cytometry, electron microscopy and proteomics. We then collected 42 samples from an additional independent cohort of PD patients to confirm our initial results. Through a series of iterative steps, we optimized an approach for defining the EV signature in PD. We found that the number of EV derived specifically from erythrocytes segregated with UPDRS scores corresponding to different disease stages. Proteomic analysis further revealed that there is a specific signature of proteins that could reliably differentiate control subjects from mild and moderate PD patients. Taken together, we have developed/identified an EV blood-based assay that has the potential to be used as a biomarker for PD.

DOI10.1016/j.nbd.2018.11.002
Alternate JournalNeurobiol. Dis.
PubMed ID30408591