Platelet abnormalities in Huntington's disease.

TitlePlatelet abnormalities in Huntington's disease.
Publication TypeJournal Article
Year of Publication2018
AuthorsDenis, HL, Lamontagne-Proulx, J, St-Amour, I, Mason, SL, Rowley, JW, Cloutier, N, Tremblay, M-È, Vincent, AT, Gould, PV, Chouinard, S, Weyrich, AS, Rondina, MT, Barker, RA, Boilard, E, Cicchetti, F
JournalJ Neurol Neurosurg Psychiatry
Date Published2018 Dec 19
ISSN1468-330X
Abstract

Huntington's disease (HD) is a hereditary disorder that typically manifests in adulthood with a combination of motor, cognitive and psychiatric problems. The pathology is caused by a mutation in the huntingtin gene which results in the production of an abnormal protein, mutant huntingtin (mHtt). This protein is ubiquitously expressed and known to confer toxicity to multiple cell types. We have recently reported that HD brains are also characterised by vascular abnormalities, which include changes in blood vessel density/diameter as well as increased blood-brain barrier (BBB) leakage.OBJECTIVES: Seeking to elucidate the origin of these vascular and BBB abnormalities, we studied platelets that are known to play a role in maintaining the integrity of the vasculature and thrombotic pathways linked to this, given they surprisingly contain the highest concentration of mHtt of all blood cells.METHODS: We assessed the functional status of platelets by performing ELISA, western blot and RNA sequencing in a cohort of 71 patients and 68 age- and sex-matched healthy control subjects. We further performed haemostasis and platelet depletion tests in the R6/2 HD mouse model.RESULTS: Our findings indicate that the platelets in HD are dysfunctional with respect to the release of angiogenic factors and functions including thrombosis, angiogenesis and vascular haemostasis.CONCLUSION: Taken together, our results provide a better understanding for the impact of mHtt on platelet function.

DOI10.1136/jnnp-2018-318854
Alternate JournalJ. Neurol. Neurosurg. Psychiatry
PubMed ID30567722