The Transgenesis platform at CRCHUL aims to facilitate research by providing transgenic services and making available a cutting edge tools and techniques.
Molecular Tools Platform
The Molecular Tools Platform located at the CERVO Brain Research Centre can assist you in the development and generation of genetic tools for your research. We have an expertise in the design and assembly of molecular constructs for expression of fluorescent markers, optogenetics and genetically modified animals. We are also specialized in the production of viral vectors (adenovirus, adeno associated virus, lentivirus, retrovirus).
Cell Sorting Platform
The CERVO Cell Sorting Platform is specialized in sorting sub-populations of neurons or cells isolated from the neuronal system. We own a FACS Aria II which physically separates various sub-populations of an heterogenic sample. We can help you with the experimental design related to cytometry and also operate the instrument for you.
Electron Microscopy Facility
The Electron Microscope Facility located at the CERVO Brain Research Centre provides advanced technical tools for transmission electron microscopy and cryofixation of biological samples. The facility offers complete processing of biological samples, including embedding and semi and ultra-thin sectioning with ultramicrotome.
The Human induced pluripotent stem cells (hiPSC) platform is an initiative of Dr. Jack Puymirat. In partnership with the Montreal Neurological Institute (MNI), under the direction of Dr. Edward A. Fon, the platform's objective is to provide access to these advanced technologies :
- Cellular reprogramming (laboratory of Dr. Jack Puymirat, CHU de Québec Research Center)
- Differentiation of hiPSCs in different neuronal types (laboratory of Dr. Thomas Durcan, MNI)
- Genome editing using the CRISPR method (laboratory of Peter McPherson, MNI)
The iPSC Quebec and iPSC / CRISPR MNI platforms work together to provide high-quality, affordable, high-quality services to academic users both in Quebec and outside, for hiPSCs reprogramming, iPSCs differentiation into different neuronal type and CRISPR genome editing.