Isolation and analysis of fractions enriched in WrappER-associated mitochondria from mouse liver.

TitleIsolation and analysis of fractions enriched in WrappER-associated mitochondria from mouse liver.
Publication TypeJournal Article
Year of Publication2021
AuthorsIlacqua, N, Anastasia, I, Pellegrini, L
JournalSTAR Protoc
Volume2
Issue3
Pagination100752
Date Published2021 09 17
ISSN2666-1667
KeywordsAnimals, Endoplasmic Reticulum, Liver, Mice, Microscopy, Electron, Mitochondria, Liver, Molecular Biology, Proteomics
Abstract

The endoplasmic reticulum (ER) plays a central role in lipid homeostasis, but the role of individual ER subdomains in lipid biology has not been elucidated. WrappER is a curved wrapping type of rough-ER that establishes extensive contacts with almost every mitochondria of the hepatocyte in the mouse liver. Here, we describe a protocol for isolation of fractions enriched in wrappER-associated mitochondria from the mouse liver. We also provide techniques for assessing its quality by electron microscopy and biochemical/proteomic analysis. For complete information on the use and execution of this protocol, please refer to Anastasia et al. (2021).

DOI10.1016/j.xpro.2021.100752
Alternate JournalSTAR Protoc
PubMed ID34458870
PubMed Central IDPMC8377487
Grant List201603PJT-365052 / / CIHR / Canada