Erythropoietin Produces a Dual Effect on Carotid Body Chemoreception in Male Rats.

TitleErythropoietin Produces a Dual Effect on Carotid Body Chemoreception in Male Rats.
Publication TypeJournal Article
Year of Publication2021
AuthorsArias-Reyes, C, Laouafa, S, Zubieta-DeUrioste, N, Joseph, V, Bairam, A, Gasser, EMSchneide, Soliz, J
JournalFront Pharmacol
Volume12
Pagination727326
Date Published2021
ISSN1663-9812
Abstract

Erythropoietin (EPO) regulates respiration under conditions of normoxia and hypoxia through interaction with the respiratory centers of the brainstem. Here we investigate the dose-dependent impact of EPO in the CB response to hypoxia and hypercapnia. We show, in isolated "" carotid body (CB) preparations containing the carotid sinus nerve (CSN) from adult male Sprague Dawley rats, that EPO acts as a stimulator of CSN activity in response to hypoxia at concentrations below 0.5 IU/ml. Under hypercapnic conditions, EPO did not influence the CSN response. EPO concentrations above 0.5 IU/ml decreased the response of the CSN to both hypoxia and hypercapnia, reaching complete inhibition at 2 IU/ml. The inhibitory action of high-dose EPO on the CSN activity might result from an increase in nitric oxide (NO) production. Accordingly, CB preparations were incubated with 2 IU/ml EPO and the unspecific NO synthase inhibitor (L-NAME), or the neuronal-specific NO synthase inhibitor (7NI). Both NO inhibitors fully restored the CSN activity in response to hypoxia and hypercapnia in presence of EPO. Our results show that EPO activates the CB response to hypoxia when its concentration does not exceed the threshold at which NO inhibitors masks EPO's action.

DOI10.3389/fphar.2021.727326
Alternate JournalFront Pharmacol
PubMed ID34594222
PubMed Central IDPMC8476757