The Lack of Amyloidogenic Activity Is Persistent in Old WT and APP/PS1ΔE9 Mouse Retinae.

TitleThe Lack of Amyloidogenic Activity Is Persistent in Old WT and APP/PS1ΔE9 Mouse Retinae.
Publication TypeJournal Article
Year of Publication2021
AuthorsJoly, S, Rodriguez, L, Pernet, V
JournalInt J Mol Sci
Volume22
Issue21
Date Published2021 Oct 20
ISSN1422-0067
KeywordsAge Factors, Alzheimer Disease, Amyloid beta-Protein Precursor, Animals, Disease Models, Animal, Electroretinography, Eye Proteins, Male, Mice, Inbred C57BL, Mice, Transgenic, Optic Nerve Injuries, Retina, Retinal Ganglion Cells
Abstract

We have previously reported that vision decline was not associated with amyloidogenesis processing in aging C57BL/6J wild-type (WT) mice and in a mouse model of Alzheimer's disease, the APP/PS1ΔE9 transgenic mouse model (APP/PS1). This conclusion was drawn using middle-aged (10-13 months old) mice. Here, we hypothesized that compared with hippocampal and cortical neurons, the weak amyloidogenic activity of retinal neurons may result in a detectable release of amyloid β (Aβ) only in aged mice, i.e., between 14 and 24 months of age. The aim of the present study was thus to follow potential activity changes in the amyloidogenic and nonamyloidogenic pathways of young (4 months) and old (20-24 months) WT and APP/PS1 mice. Our results showed that in spite of retinal activity loss reported by electroretinogram (ERG) recordings, the level of amyloid beta precursor protein (APP) and its derivatives did not significantly vary in the eyes of old vs. young mice. Strikingly, the ectopic expression of human APP in APP/PS1 mice did not allow us to detect Aβ monomers at 23 months. In contrast, Aβ was observed in hippocampal and cortical tissues at this age but not at 4 months of life. In contrast, optic nerve transection-induced retinal ganglion cell injury significantly affected the level of retinal APP and the secretion of soluble APP alpha in the vitreous. Collectively, these results suggest that the amyloidogenic and nonamyloidogenic pathways are not involved in visual function decline in aging mice. In WT and APP/PS1 mice, it is proposed that retinal neurons do not have the capacity to secrete Aβ in contrast with other cortical and hippocampal neurons.

DOI10.3390/ijms222111344
Alternate JournalInt J Mol Sci
PubMed ID34768774
PubMed Central IDPMC8583210