Erythropoietin in the Locus coeruleus attenuates the ventilatory response to CO2 in rats.

TitleErythropoietin in the Locus coeruleus attenuates the ventilatory response to CO2 in rats.
Publication TypeJournal Article
Year of Publication2017
AuthorsSilva, CA, Vicente, MC, Tenorio-Lopes, L, Soliz, J, Gargaglioni, LH
JournalRespir Physiol Neurobiol
Volume236
Pagination11-18
Date Published2017 02
ISSN1878-1519
Abstract

The Locus coeruleus (LC) is a pontine area that contributes to the CO2/pH chemosensitivity. LC cells express erythropoietin (Epo) receptors (EpoR), and Epo in the brainstem is a potent normoxic and hypoxic respiratory stimulant. However, a recent study showed that the intra-cisternal injection (ICI) of Epo antagonist does not alter the hypercapnic ventilatory response in mice. As ICI leads to a widespread dispersal of the product throughout the brainstem, in this work we evaluated the specific impact of Epo in the LC-mediated ventilatory response to CO2 (by whole body plethysmography) in juvenile male Wistar rats. Normocapnic and hypercapnic ventilation were evaluated before and after unilateral microinjection of Epo (1ng/100nL) into the LC. To evaluate the long-term effect of Epo, the HcVR was re-evaluated 24h later. Our results show that Epo attenuates the hypercapnic ventilation. We conclude that Epo in the LC tunes the hypercapnia-induced hyperpnea.

DOI10.1016/j.resp.2016.10.011
Alternate JournalRespir Physiol Neurobiol
PubMed ID27818313