Effects of Dietary Bisphenol A on the Reproductive Function of Gilthead Sea Bream () Testes.

TitleEffects of Dietary Bisphenol A on the Reproductive Function of Gilthead Sea Bream () Testes.
Publication TypeJournal Article
Year of Publication2019
AuthorsForner-Piquer, I, Fakriadis, I, Mylonas, CC, Piscitelli, F, Di Marzo, V, Maradonna, F, Calduch-Giner, J, Pérez-Sánchez, J, Carnevali, O
JournalInt J Mol Sci
Volume20
Issue20
Date Published2019 Oct 10
ISSN1422-0067
KeywordsAnimal Feed, Animals, Benzhydryl Compounds, Body Weight, Diet, Endocannabinoids, Endocrine Disruptors, Estradiol, Gene Expression Regulation, Gonadal Steroid Hormones, Gonads, Lipogenesis, Liver, Male, Models, Animal, Phenols, Receptors, Leptin, Reproduction, Sea Bream, Sperm Motility, Spermatozoa, Testis, Testosterone, Transcriptome, Up-Regulation, Vitellogenins, Zona Pellucida
Abstract

Bisphenol A (BPA), a known endocrine disrupting chemical (EDC), was administered by diet to gilthead sea bream () in order to study its effects on the endocannabinoid system (ECS) and gonadal steroidogenesis. 2-year-old male gilthead sea bream were fed with two different concentrations of BPA (LOW at 4 and HIGH at 4000 µg/kg body weight for 21 days during the reproductive season. Exposure to 4000 µg BPA/kg bw/day (BPA HIGH) reduced sperm motility and altered the straight-line velocity (VSL) and linearity (LIN). Effects on steroidogenesis were evident, with testosterone (T) being up-regulated by both treatments and 11-ketotestosterone (11-KT) down-regulated by BPA HIGH. Plasma levels of 17β-estradiol (E) were not affected. The Gonadosomatic Index (GSI) increased in the BPA HIGH group. Interestingly, the levels of endocannabinoids and endocannabinoid-like compounds were significantly reduced after both treatments. Unpredictably, a few changes were noticed in the expression of genes coding for ECS enzymes, while the receptors were up-regulated depending on the BPA dose. Reproductive markers in testis (leptin receptor (), estrogen receptors (, ), progesterone receptors () and the gonadotropin releasing hormone receptor ()) were up-regulated. BPA induced the up-regulation of the hepatic genes involved in oogenesis (vitellogenin () and zona pellucida 1 ()).

DOI10.3390/ijms20205003
Alternate JournalInt J Mol Sci
PubMed ID31658598
PubMed Central IDPMC6835794
Grant Listprot 2010W87LBJ / / Ministero dell'Istruzione, dell'Università e della Ricerca /
652831 / / Horizon 2020 /