Apolipoprotein E isoforms disrupt long-chain fatty acid distribution in the plasma, the liver and the adipose tissue of mice.

TitleApolipoprotein E isoforms disrupt long-chain fatty acid distribution in the plasma, the liver and the adipose tissue of mice.
Publication TypeJournal Article
Year of Publication2014
AuthorsConway, V, Larouche, A, Alata, W, Vandal, M, Calon, F, Plourde, M
JournalProstaglandins Leukot Essent Fatty Acids
Volume91
Issue6
Pagination261-7
Date Published2014 Dec
ISSN1532-2823
KeywordsAdipose Tissue, Animals, Apolipoprotein E2, Apolipoprotein E3, Apolipoprotein E4, Apolipoproteins E, Carnitine O-Palmitoyltransferase, Fatty Acid Transport Proteins, Fatty Acid-Binding Proteins, Fatty Acids, Fatty Acids, Omega-3, Female, Genotype, Humans, Liver, Male, Mice, Mice, Inbred C57BL, Protein Isoforms
Abstract

Evidences suggest that omega-3 fatty acid (n-3 PUFA) metabolism is imbalanced in apolipoprotein E epsilon 4 isoform carriers (APOE4). This study aimed to investigate APOE genotype-dependant modulation of FA profiles, protein and enzyme important to fatty acid (FA) metabolism in the adipose tissue, the liver and the plasma using human APOE-targeted replacement mouse-model (N=37). FA transport (FATP) and binding (FABP) protein levels in tissues and concentrations of liver carnitine palmitoyltransferase 1 (CPT1) were performed. N-3 PUFA concentration was >45% lower in the adipose tissue and liver of APOE4 mice compared to APOE3 mice. In APOE4 mice, there were higher levels of FATP and FABP in the liver and higher FATP in the adipose tissue compared to APOE2 mice. There was a trend towards higher CPT1 concentrations in APOE4 mice compared to APOE3 mice. Therefore, since APOE-isoform differences were not always in line with the unbalanced n-3 PUFA profiles in organs, other proteins may be involved in maintaining n-3 PUFA homeostasis in mice with different APOE-isoforms.

DOI10.1016/j.plefa.2014.09.007
Alternate JournalProstaglandins Leukot. Essent. Fatty Acids
PubMed ID25301204
Grant ListMP-MOP119454 / / Canadian Institutes of Health Research / Canada